Answer:
Its D mitochondria
Explanation:
lol i learned about cells for a longggg time so i know this
Consider our yeast CRISPR-Cas9 experiment: yeast are transformed with the PML 104-9RNA1 plasmid + HDR1 template to edit the ADE2 gene. Enter your answers below.
a. In yeast, what is the template from which the gRNA is transcribed?
b. The HDR fragment must enter the of the yeast cell to be used for homology directed repair.
c. The Cas9 gene is transcribed from the ____________as template, the transcript is transported from the _______to the ____________of the cell, and then the protein is translated.
d. After translation of Cas9, what happens? Explain in ONE sentence.
Answer:
a. The guide RNA (gRNA) is transcribed from the PML 104-9RNA1 plasmid vector
b. It is correct. The homology-directed repair (HDR) fragment of interest enters into the cell and is then inserted at the site of the original double-strand breaks (DSBs) via the HR DSB repair pathway.
c. plasmid, nucleus, cytoplasm
d. Once Cas9 is transcribed, this nuclease forms a complex with the gRNA and cuts the target DNA, thereby inducing a DNA double-strand break (DSB) which is repaired either by non-homologous end joining (NHEJ) or homology-directed repair (HDR)
Explanation:
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (i.e., CRISPR-associated protein 9) is a natural prokaryotic defense mechanism against foreign viral invaders. In genome engineering, this system has been adapted to create a versatile RNA-programmable genome editing technique. Cas9 is an RNA-guided endonuclease capable of recognizing and cleaving a target DNA complementary to the guide RNA (gRNA). The gRNA is a specific RNA sequence formed by two small RNAs (crRNA and tracrRNA), which binds to the Cas nuclease enzyme and guides the gRNA-Cas9 complex to the target DNA sequence.
How do petals help with fertilization
he G-protein-linked receptor is located ___________________. When GDP is attached to the G protein the messenger is considered _______. GTP replaces GDP and now the messenger is considered _______. The G protein carrying the GTP leaves the receptor and _______ and enzyme which causes a cellular response. All of this is brought on by a _______ ________ attaching to the G-protein-linked receptor and will shut down quickly when the ___________ ____________ is no longer there.
Answer:
located... on the plasma membrane
is considered... inactive
the messenger is considered... to be active
All of this is brought on by a.... signal molecule
will shut down quickly when the.... signal molecule
Explanation:
The G protein-coupled receptors (GPCRs) are transmembrane proteins found on the plasma membrane. GPCRs are considered to be the most diverse group of transmembrane receptors in eukaryotic cells. These proteins (GPCRs) bind to signaling molecules to trigger signaling pathways through Guanine nucleotide-binding proteins (G-proteins). G proteins are signaling proteins (for example, Ras protein) that bind both GTP and GDP. After the signaling molecule binds to GPCR, GTP is hydrolyzed by the G-protein in a process that triggers specific signaling pathways (for example, activation of the production of second messengers).
what is anther term for muscle cell
Answer:
myocyte
Explanation:
Ultraviolet radiation is harmful O because itcauses unchecked photosynthesis.
O damages DNA and protein.
O penetrates the aphotic zone.
O none of the above
how do waves affect landforms
the pressure and the force of waves produce more rocks
pressure and force of ways can break Cliffs Apart
waves can cause rocks to change it what it is made of
Answer:
Waves erode the bedrock along the coast largely by abrasion. The suspended sediment particles in waves, especially pebbles and larger rock debris, have much the same effect on a surface as sandpaper does. Waves have considerable force and so may break up bedrock simply by impact.
What does the pH of a solution measure?
Answer:
It measures how acidic or water-based something is.
Explanation:
The explanation is the answer
Answer:
the acidity or alkilinity of a substance is measured by ph scale
1-7 is an acid and 7-14 is a base
i hope this helps
can i have brainliest
stay safe
Does binary fission use mitosis
Answer:
Yes, binary fission is an example of mitosis.
Explanation:
Once all the members of a ____ have this favorable traits then it is refferred to as an _____
Answer:
Once all the members of a population have this favorable trait, then it is referred to as an adaptation.
Explanation:
In which biome would you expect to find fire and drought resistant plants? A) savanna B) decidious forests C) rainforest D) tundra E) alpine
Answer:
A. savanna
Explanation:
because its a desert and all the plants there are made for the heat such as cacti
explain the life cycle of a Diploid cell,
ASAP
Which discovery supported the endosymbiotic theory?
The discovery of independent DNA (from the host) in mitochondria and chloroplasts supports the endosymbiotic theory.
The endosymbiotic theory is the theory that mitochondria and chloroplasts, two important organelles found in eukaryotic cells, originated from bacteria that were engulfed by larger cells.
What is this theory?The presence of DNA in mitochondria and chloroplasts. Mitochondria and chloroplasts have their own DNA, which is separate from the DNA in the nucleus of the cell. This suggests that they were once independent organisms.
The similarity of mitochondrial and chloroplast DNA to bacterial DNA. The DNA in mitochondria and chloroplasts is similar to the DNA of bacteria. This suggests that they share a common ancestor.
The presence of ribosomes in mitochondria and chloroplasts. Mitochondria and chloroplasts have ribosomes, which are structures that are used to make proteins. Ribosomes are also found in bacteria.
Find out more on endosymbiotic theory here: https://brainly.com/question/1698852
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The first hour bell rings stimulus or response?
Answer:
The ringing of the bell was Introduced as a neutral
stimulus by Pavlov. A stimulus which leads to an
automatic response is an unconditioned stimulus.
The food was the unconditioned trigger in Pavlov's
experment. An automatic response to a stimulus is an unconditioned response.
If it's not to much to ask, can you please tell me which one goes on the line please and thank u
Answer:
Cell theory
Explanation:
Which criteria does the student use as the basis for the classification
Answer:
what is that supposed to mean
Explanation:
Answer:
the main criteria for classifying organism into five kingdoms are the complexity of cells structure, body organization, the mode of nutrition, life style, and phylogenetic relationship. This system consists Monera, Protista, Fungi, Plantae, and Animalia.
Explanation:
Im pretty sure
PLEASE HELP! You have been infected with the H1N1 flu virus. Describe how this virus might have entered your body, how it works to make you sick, and what happens when you finally get better. List at least 7 different steps
Answer:
Eat ginger!
Explanation:
Hope this help!
maple trees lose their leaves in fall. which part of carbon cycle comes next
On falling to the ground they mix with the soil with the help of bacteria. The carbon is released from the leaves in the air and is added to the carbon cycle.
What is the carbon cycle. ?The maple tree is a shrub that is commonly called maple. They are 132 species, that are native to Asia, appear in Europe, Africa and north and south America.
The carbon cycle is a process by which the carbon is returned back to the air and it continuously travels back to earth. After the fall the leaves fall down to the ground and re-merged to the soil as humus. The bacteria converts the energy that is again taken up by the plants.
Find out more information about the leaves in the fall.
brainly.com/question/5052077.
Answer:
Decomposition
Explanation:
When DNA replicates, each strand of the original DNA molecule is used as a template for the synthesis of a second, complementary strand. Compare and contrast the replication of the two new strands, listing and explaining at least one similarity and one difference in the methods of synthesis.
Answer:
See the answer below
Explanation:
A typical double-stranded DNA consists of a leading and a lagging strand with 5' to 3' direction and 3' to 5' direction respectively. During replication, a RNA primer binds to each strand at the replication origin and DNA polymerase enzyme adds complementary nucleotides to the primer to elongate the new strand until the end of each strand is reached.
The replication process of the two strands are similar such that:
Both require RNA primer bindingBoth require DNA polymerase to add nucleotides in 5' to 3' directionThe replication of the two strands are different in a certain way:
The enzyme responsible for adding nucleotide bases to the growing DNA strand during replication can only add in 5' to 3' direction. Consequently, the leading strand is synthesized in a continuous manner while the lagging strand is synthesized discontinuously in short nucleotide sequences known as the Okazaki fragments. The fragments are later joined together by an enzyme known as DNA ligase.This means that the RNA primer only binds at a single location on the leading strand while multiple primers bind at multiple locations on the lagging strand. The replication of the twoHelp me asapppp.............
Good luck, I have no clue
a group of students tested to see if the acidity of water affected pea plant growth
Answer:
I would say C because in number one, the tests were never repeated
Explanation:
do you mean
this question??
Experiment 1: A group of students tested to see if the acidity of water affected pea plant growth. The students followed a logical method and performed their experiment once, using control and experimental groups. Their results supported their hypothesis.
Experiment 2: A group of students tested to see if bacteria growth in water was affected by acidity. The students used control and experimental groups. They repeated their experiment several times and found the results were the same.
Which experiment most likely has greater reliability?
A. Number 1 because it follows a logical method
B. Number 2 because the experiment was repeated
C. Number 2 because the experiment was repeated and the results were always the same
D. Number 1 because it has control groups
What is the answer to this question?
*If you get it right you get brainliest.*
PLZ HELP
Answer:
It's D)Engineering
Explanation:
Hope this helps out :)
What could people change that would reduce the burning of fossil fuels?
could use less heat and air conditioning, lighting, electronics in our homes. Drive gasoline cars less often, and walk more. stop buying new clothes as much, and recycling used. use renewable energy sources like wind and solar power.
How are the human body and a machine alike?
Answer:
All muscles differ from each part of the body. Motion is all around us. The human body has developed the forces that act on it, making a human machine that moves effectively and efficiently. Machines are made to help humans function better.
Explanation:
The bright tail of a comet consists of _____.
ice particles
gas vapors
rock fragments
dust
Answer:
dust is the correct answer
What role does the pollen tube play in fertilization
What life process builds large complex substances from smaller, simpler substances?
A. Regulation
B. Growth
C. Synthesis
D. Excretion
Put each step in the correct place in this scientific investigation.
Answer: What is the order of steps in the scientific method?
The scientific method has five basic steps, plus one feedback step:
Make an observation.
Ask a question.
Form a hypothesis, or testable explanation.
Make a prediction based on the hypothesis.
Test the prediction.
Iterate: use the results to make new hypotheses or predictions.
HOPE THIS HELPS
During the filtration process of urine, the glomerulus filters water and other dissolved substances from
A)
bile
B)
blood
C)
semen
D)
water
Answer: Blood
Explanation: This is the actual answer for USATestprep i already checked the answer it isnt bile
Which of these is NOT a forest ecosystem service?
A Stores carbon dioxide
B Absorbs and releases water
C Increases erosion
D Nutrient Cycling
Answer:
Nutrient Cycling
Explanation:
which of the following best explains the purpose of photosynthesis?
In photosynthesis, oxygen is used to make sugar.
In photosynthesis, sunlight is used to make ATP
in photosynthesis, sunlight is used to make sure
in photosynthesis glucose is used to make energy
Answer:
Plants are autotrophs, which means they produce their own food. They use the process of photosynthesis to transform water, sunlight, and carbon dioxide into oxygen, and simple sugars that the plant uses as fuel. These primary producers form the base of an ecosystem and fuel the next trophic levels.
Explanation:
