Consider our yeast CRISPR-Cas9 experiment: yeast are transformed with the PML 104-9RNA1 plasmid + HDR1 template to edit the ADE2 gene. Enter your answers below.
a. In yeast, what is the template from which the gRNA is transcribed?
b. The HDR fragment must enter the of the yeast cell to be used for homology directed repair.
c. The Cas9 gene is transcribed from the ____________as template, the transcript is transported from the _______to the ____________of the cell, and then the protein is translated.
d. After translation of Cas9, what happens? Explain in ONE sentence.

Answers

Answer 1

Answer:

a. The guide RNA (gRNA) is transcribed from the PML 104-9RNA1 plasmid vector

b. It is correct. The homology-directed repair (HDR) fragment of interest enters into the cell and is then inserted at the site of the original double-strand breaks (DSBs) via the HR DSB repair pathway.

c. plasmid, nucleus, cytoplasm

d. Once Cas9 is transcribed, this nuclease forms a complex with the gRNA and cuts the target DNA, thereby inducing a DNA double-strand break (DSB) which is repaired either by non-homologous end joining (NHEJ) or homology-directed repair (HDR)

Explanation:

The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (i.e., CRISPR-associated protein 9) is a natural prokaryotic defense mechanism against foreign viral invaders. In genome engineering, this system has been adapted to create a versatile RNA-programmable genome editing technique. Cas9 is an RNA-guided endonuclease capable of recognizing and cleaving a target DNA complementary to the guide RNA (gRNA). The gRNA is a specific RNA sequence formed by two small RNAs (crRNA and tracrRNA), which binds to the Cas nuclease enzyme and guides the gRNA-Cas9 complex to the target DNA sequence.


Related Questions

Questlon 4 of 10
Why is it important for scientists to state a conclusion?
OA. To compare their hypothesis to the data that they observed
O B. To make an educated guess about an event
OC. To appear reliable to other scientists
O D. To clearly state why the experiment was performed
SUBMIT

Answers

A is the answer to the equation.

Identify the step in which water changes from a liquid to a gas.

Answers

The step in which water changes from a liquid to a gas is evaporation.

Hope it helps!

Where is the protective covering of the axon?

Answers

Answer:

Myelin. a fatty substance that forms a protective coating around the axon of a neuron

Explanation:

myelin sheath
The axon is covered by a myelin sheath. The axon passes information to the next neuron or other cell in the body. A myelin sheath. The axons have a protective covering called myelin.

Which phrase describes air density?
O increases as altitude increases
O equals mass divided by volune
O pushes molecules in one direction
O exets less pressure as it increases

Answers

Answer:

Explanation:

equals mass divided by volume

exerts more pressure as it increases

measures how tightly molecules are packed

Answer: The answer is: equals mass divided by volume

Explanation: I have my ways ;>

When does consumption begin to surpass supply?
A. In about 200 years
B. In about 700 years
C. In about 550 years
D. In about 625 years

Answers

Answer:

625 years

Explanation:

Label the photo below.​

Answers

Answer:

left is red blood cell (erythrocyte)

bottom is platelet (thrombocyte)

right is white blood cell (leukocyte)

Does the temperature increase during melting?
Explain why

Answers

Answer:

Melting is the process where the atoms of a rigid solid Crystal lattice held by strong bonds, are broken down which increases the degree of locomotion of these atoms which characterises the liquid state.

The energy that breaks these bonds is heat energy ( latent heat of fusion ), hence temperature increases

NEED HELP BAD!!!!!!!!!! Its on edmentum,but im sure plato answers are correct too!! PLEASE HELP
In what way are the humerus and the femur similar?

an x-ray of the human body showing the humerus and femur bones

A.
They both attach their respective limbs to the axial skeleton.
B.
They both have similar joints at each end.
C.
They both are extremely strong and made only of compact bone.
D.
They both have hollow spaces filled with bone-generating cells.
E.
They both protect vital organs.

Answers

Answer:

b

Explanation:

just took the test on plato and got a 5/5

Question 3 of 10
What is the next step in the scientific method, following forming a
hypothesis?
OA. Analyzing the data
O B. Stating the question
OC. Conducting an experiment
SUBMIT

Answers

C: conducting an experiment

Which one of these is NOT an example of intercellular communication?

A. Electrical signals pass between heart cells

B. Chemical messengers doc on two receptors of adrenal gland

C. A vessel and encloses over a nutrient molecule

D. Chemical signals from pituitary gland to adrenal gland

Answers

Answer:

A. Electrical signals pass between heart cells

What is the importance of testicular self exam

Answers

Answer:

Testicular self-exam is important because it helps detect abnormalities, especially cancer. Physicians don't check the testicles as much, so it is important to self-exam more often. Testicular cancer is more likely to be found if checked monthly. Besides cancer, routine exams create a feel for the characteristics of the testicles and would be easier to detect a sudden change.

Explanation:

Science experiment : What can you fit deep in your throat?

Answers

Answer:

I don't no this answer

so sorry

⚠️ ⚠️ Help Me Please Activity A. ??????!!!!?!!

Answers

Answer:

1. Nucleus ---> present in both plant and animal cells

Cell membrane ---> present in both plant and animal cells

Cytoplasm ---> present in both plant and animal cells

Golgi apparatus ---> present in both plant and animal cells

Mitochondria ---> present in both plant and animal cells

Vacuole ---> present in both plant and animal cells

Ribosome ---> present in both plant and animal cells

Endoplasmic reticulum ---> present in both plant and animal cells

Lysosome ---> present in animal cells only

Nucleolus ---> present in both plant and animal cells

Explanation:

2. The common organelles present in both plant and animal cell include the nucleus, cytoplasm, cell membrane, mitochondria, ribosome, etc. The presence of these common organelles is due to the fact that that these organelles help both plants and animal cells to carry out the basic functions of life. Also, plant and animals cells are both eukaryotic cells.

3. Plant cell differ from animal cells due to some specialized functions they carry out like photosynthesis, as well as some structural differences in plant cells. Plants cells have chloroplasts which are organelles responsible for photosynthesis in plants. Plant cells also have a large central vacuole unlike animal cells which help to maintain turgidity in plants. Cellulose cell wall in plant cell also serve to maintain the rigidity and give shape to the plant as well as to protect the plant cell.

Explain where the carbon stored in fossil fuels comes from.

Answers

Answer: Rocks like limestone and fossil fuels like coal and oil are storage reservoirs that contain carbon from plants and animals that lived millions of years ago. When these organisms died, slow geologic processes trapped their carbon and transformed it into these natural resources.

Explanation:

where are coal, oil, natural gas commonly found

Answers

Answer:

Saudi Arabia, Russia, the United States, and Iran

Explanation:

How do all body systems work together when you touch a thorn?

Answers

A reflex action is a way for the body to automatically and rapidly respond to a stimulus to minimise any further damage to the body.

Answer:

first nervous system do transfer of signal then nervs say muscle to contract and we put our hand back

Which of the following is NOT an example of a cnidarian?

Question 3 options:

anemone


jellyfish


snail


coral

I will mark who ever has this correct Brainliest ( for this question the points will be 85 )

Answers

I’d say it is a snail. I know coral and jellyfishes are definitely cnidarians as well as anemones. I’m really sorry if I’m wrong but I really think it is a snail.

From the following, the example which is not of a cnidarian is Snail. Hence, the correct option is C.

What are Cnidarians?

Cnidarians are the radially symmetrical soft bodied animals that belong to phylum Cnidaria of the animal kingdom. They are also called coelenterates. The snails belong to phylum mollusca.

They are all sea dwelling animals which have a hollow digestive cavity. Furthermore, they appear in either sessile polyp stage or moving medusa stage. Moreover, they are further divided in classes Schyphozoa, Anthozoa, Hydrozoa and Cubozoa. All having different characteristics.

They show both sexual and asexual reproduction. Also, their development is indirect, meaning the development takes place from a larval stage. They show the property of polymorphism and have cnidoblasts cells for defense. Hence, the correct option is C.

For more details regarding cnidarians, visit:

https://brainly.com/question/28321349

#SPJ6

Select the correct the answer.
Which process will decrease the level of CO2 in the atmosphere?
O A. growing trees
OB.
cutting trees
O C.
burning trees
OD.
increasing the human population

A is the answer

Answers

Answer:

A. Growing trees

Explanation:

I majored in Biology

What do proteins do in the body?

A. Make up cell membranes
B. Make sugars
C. Do work
D. Store energy

Answers

Answer: A

Explanation:
Proteins make up cell membranes.

Hope this helps! ^^

When problem solving and making decisions, your rational thought is
centered in your
O A. temporal lobe.
O B. prefrontal cortex.
O c. midbrain.
O D. occipital lobe.

Answers

Answer:

b

Explanation:

that is the answer thanx

please help! I will mark brainliest
Which characteristic do euglenoids and algae share?
A. Both are fungus-like protists.
B. Both can be autotrophic.
C. Both have whip-like tails.
D. Both are decomposers.​​

Answers

Answer:

the answer is both are autotrophic

Explanation:

I got the answer right just now

Answer:

B.) Autotrophic

Explanation:

B Euglenoids and algae both can be autotrophic.

Hope this helps!!

The complete sequence of nucleotides in the human genome would fill more than ___________ books!

Answers

Answer:

The complete sequence of nucleotides in the human genome would fill more than 3000 books.

Explanation:

Genome corresponds to the set of genes that determine the morphological and functional characteristics of living beings.

In the human, the genome consists of the total sequence of nucleotides found in the DNA, contained in the cell nucleus, and distributed in the 23 pairs of chromosomes it possesses.

In the nucleus of a human cell there are about 3,200,000,000 DNA nucleotides, without taking into account the nucleotides of the mitochondrial genome, just over 15,000.

According to this, the amount of books that could be filled with the information of the human genome is estimated at 3,000 books.

Which of the following cell types is formed by meiosis? A. muscle cells B . sperm cells C. skin cells D. blood cells

Answers

Answer: B

Explanation: The rest are produced through mitosis.

A type of cell that is created during the process of meiosis is known as a sperm cell. Thus, the correct option is C.

What is Meiosis?

Meiosis can be defined as the type of the cell division which goes through or in which two daughter cells has been produced from the parental cell that has not genetically identical to their real parental cell. Meiosis has been also known as the reductional division.

The germ cells must be generally produced by the process of the meiosis. It is because these cells has been usually contain half of the amount of the genetic material as it is compared to the parental cell. After producing these gametes, the cells has undergo through the process of the fertilization in order to produce the product known as zygotes which contain the genetic material of both males and females.

Therefore, sperm cells and egg cells are the types of cells that are created during the process of meiosis.

To learn more about Meiosis, refer to the link:

brainly.com/question/8253366

#SPJ3

Practice the above directional terms by describing the following relationships: The trachea (windpipe) is ___________ to the esophagus (throat). Hair is ___________ to muscle. The thumb is ___________ to the ring finger. The heart is ___________ to the lungs. The ear is ___________ to the eye. The abdomen is ___________ to the thorax. Thigh muscles are ___________ to the femur (thigh bone). Skin is ___________ to skeletal muscles. The shoulder is ___________ to the elbow. The hand is ___________ to the elbow. The rib cage is ___________ to the upper arm.

Answers

Answer:

Practice the above directional terms by describing the following relationships: The trachea (windpipe) is anterior to the esophagus (throat). Hair is superficial to muscle. The thumb is lateral to the ring finger. The heart is medial to the lungs. The ear is lateral to the eye. The abdomen is inferior to the thorax. Thigh muscles are superficial to the femur (thigh bone). Skin is superficial to skeletal muscles. The shoulder is proximal to the elbow. The hand is distal to the elbow. The rib cage is lateral to the upper arm.

Explanation:

Based on the information in the stimulus materials, which of the following choices explains why domesticated horses and takhi horses have different body styles? Select two answers.

Select one or more:
A. The takhi horse’s genes mutated in the 1960’s causing it to appear different than domesticated horses.
B. Humans have been breeding the takhi horse for hundreds of years to exhibit certain traits.
C. The takhi horse has adapted tothe environment of captivity.
D. Humans have been breeding the domesticated horse over thousands of years to exhibit certain traits.
E. The takhi horse has adapted to the environment of the Mongolian steppe.

Answers

Answer:

a b e

Explanation:

True or False: When you grow the cells in your body stay the same size, but they multiply in number by growing and dividing.

Answers

Answer: The answer is generally no, but sometimes yes.


Explanation: We grow to our full adult size via an increase in the number — not the size — of our cells. But some of our cells can change size and this can be for healthy or not-so-healthy reasons.

Hope this helps! ^^

Which of the following is an examples of a species:

A:lions and tigers

B: poodles and golden retrievers

C:Horses and zebras

D:Horses and donkeys

Answers

Answer:

C Horses and Zebras

Explanation:

Zebras are a different species of a horse that lives in Africa.

Answer is C Horses and Zebras

hey lol please help I have like 192793 missing assignments

Answers

Answer:

Chromosomes are structures within cells that contain a person's genes. Genes are contained in chromosomes, which are in the cell nucleus.Explanation: Chromosomes are structures within cells that contain a person's genes. Genes are contained in chromosomes, which are in the cell nucleus. ... Every normal human cell contains 23 pairs of chromosomes, for a total of 46 chromosomes. A trait is any gene-determined characteristic and is often determined by more than one gene.

Which are parts of the respiratory system?

artery
trachea
vein
lung
***ANSWER: Trachea and lung***​

Answers

Answer:

- Bronchial Artery

- Lung

-Trachea

Explanation:

Hope this helps!

p.s: The veins are part of the circulatory system.

Answer:

the person that asked the question is right

Explanation:

B) trachea

D) lung

when moving to a higher power what must you NOT do?? (microscopes)

Answers

Answer:

make sure the microscope lens isnt touching the sample thingy

Explanation:

always have a partner telling you when to stop zooming in to avoid damage to the microscope

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